用鲁米诺功能化金纳米粒子修饰的金电极电化学发光法测定多巴胺

Electrochemiluminescence Determination of Dopamine Using Luminol Functionalized Gold Nanoparticles Modified Gold Electrode

摘要: 采用鲁米诺功能化金纳米粒子修饰的金电极制备了新型的电化学发光传感器(ECLS),并对其电化学发光(ECL)性质作了研究。结果表明:该修饰电极具有良好的ECL性能,在含有1.0×10-3mol·L-1过氧化氢的0.02 mol·L-1碳酸钠-碳酸氢钠缓冲溶液(pH 10.0)中,其ECL强度值与多巴胺浓度的对数值在1.0×10-10~1.0×10-5mol·L-1范围内呈线性关系,检出限(3σ)为6.3×10-11mol·L-1。据此,应用该传感器测定了尿样中的多巴胺含量,并对方法的回收率作了试验,测得平均回收率为103%。

Abstract: Using the Au-electrode modified with luminol functionalized gold nanoparticles, a novel electrochemiluminescent sensor (ECLS) was prepared, and its ECL behavior was studied. As shown by the experimental results, excellent ECL performance was displayed by the modified electrode, and when the novel ECLS was used in a 0.02 mol·L-1 Na2CO3-NaHCO3 buffer solution (pH 10.0) containing 1.0×10-3mol·L-1 H2O2, linear relationship between values of ECL intensity and logarithm of concentration of dopamine was obtained in the range of 1.0×10-10-1.0×10-5mol·L-1 of dopamine, with detection limit (3σ) of 6.3×10-11mol·L-1. Based on these facts, the ECLS was used in the determination of dopamine in urine samples, and recovery of the method was tested, giving average recovery of 103%.